FastQCFastQC Report
Sat 18 Mar 2023
SRR4895655_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895655_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences330288
Sequences flagged as poor quality0
Sequence length151
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGAGCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT105203.1850990650583735No Hit
CTGAGCCATGTACTCTGCGTTGATACCACTGCTTCCCACGTACTCTGCGT12400.3754299278205687No Hit
CTGAGCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTT11050.3345565082594584No Hit
CTGAGCCAATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG4230.1280700479581456No Hit
CTGAGCCATGTACTCTGCGTTGAGACCACTGCTTCCCATGTACTCTGCGT3730.11293174441699366No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATACGA253.8287908E-8144.209537
AGTTCGA100.0072055277144.209538
GAGCCAT129200.0144.042113
TGAGCCA330650.0144.035082
CTGAGCC330900.0143.926251
GCCATGT36150.0143.41175
AGCCATG60250.0143.132454
GCCAATG11650.0142.971685
GAGCCAG103500.0142.955553
GAGCCAA92450.0142.649673
AGCCAGT36900.0142.646294
AGCCAAT32350.0142.426424
AGCCAGA31600.0141.927754
AGCCATA34300.0141.897144
GCCAGAT11550.0141.71245
AGCCATT34650.0141.296224
AGCCAAG30950.0140.249014
CCAGTGA3500.0140.089266
GCCAGTA12150.0140.055345
GCCATTA8500.0139.11985
Produced by FastQC (version 0.11.5)