FastQCFastQC Report
Sat 18 Mar 2023
SRR4895655_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895655_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences330288
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG262067.934287651988567No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA38061.1523276655524874No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11800.35726396357118634No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA6720.20345879959308238No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG6290.19043985854769171No Hit
AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA4710.1426028193576515No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC3580.10839025335464808No Hit
AGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG3510.10627089085888679No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGCTCAGAGATCGGAAGAGCACA3370.10203216586736423No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT199150.067.740411
AGCAGTG206350.065.351562
CAGTGGT218700.061.628014
AGTGGTA218700.061.5617075
GCAGTGG219800.061.187653
GGTATCA222500.060.8779688
GTATCAA222950.060.778419
GTGGTAT221900.060.641266
TGGTATC223200.060.2971887
CGATAAG604.943395E-448.327072145
GGGGTAT2052.910383E-1035.366636
GGTCTGA1458.6837827E-734.99547145
AGGGGTA1707.794188E-834.1183975
CCGCAAG1501.1319862E-633.828953145
TTGTCGT605.875336E-1026.58472120-124
TACGGAA1100.00966848926.360224145
CCGTCTT6550.025.454718145
CCGGCTT2056.4125306E-624.752893145
GGGTATC2451.9027557E-623.6775747
CATGGGC14800.023.51047145
Produced by FastQC (version 0.11.5)