FastQCFastQC Report
Sat 18 Mar 2023
SRR4895654_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895654_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1500239
Sequences flagged as poor quality0
Sequence length151
%GC34

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCAATG1502000.0144.042071
TCAATGA1503000.0143.946242
CAATGAG508950.0143.81823
CAATGAT537450.0143.32943
CAATGAA440200.0142.576843
AATGATG202800.0139.663744
AATGAGT184450.0139.562824
AATGAGG191100.0137.121124
AATGAAG164150.0136.703264
AATGAGA151150.0136.15264
AATGATA168700.0134.725974
CAATGAC24950.0134.677663
AATGAAT167300.0133.138024
AATGATT192550.0131.482354
ATGATGA61000.0130.85735
ATGATAG64650.0130.719155
ATGAGTA63050.0130.491065
ATGATGG73700.0130.419595
ATGAGTG60550.0130.162575
ATGAATG59700.0128.271565
Produced by FastQC (version 0.11.5)