FastQCFastQC Report
Sat 18 Mar 2023
SRR4895654_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895654_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1500239
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG336832.2451756020207445No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA74060.49365467768802174No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA45640.3042181945676656No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG21360.14237731454788202No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC16730.1115155651866136No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT401600.084.045991
AGTGGTA406150.082.7692955
GGTATCA410500.082.1013958
GTGGTAT410400.081.891766
AGCAGTG412700.081.642212
GTATCAA415900.081.122569
CAGTGGT415900.080.857144
TGGTATC417500.080.516487
GCAGTGG422400.079.527063
CGGTCTG5400.053.7031145
CGATAGG2900.023.9994135-39
GGTCTGA10900.023.944685145
TACGCAC4400.023.3960650-54
CATGGGG83950.022.7990325-29
CGCGAAA5750.022.19194290-94
CGACCTA4800.021.44624545-49
CCGCGAA6100.020.91863490-94
GTTCTCG6500.019.853622110-114
GGTCGCG5200.019.79666550-54
CTCGTTG5950.019.49531735-39
Produced by FastQC (version 0.11.5)