FastQCFastQC Report
Sat 18 Mar 2023
SRR4895653_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895653_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences736895
Sequences flagged as poor quality0
Sequence length151
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCATACATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT42560.5775585395476968No Hit
CGCATACATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTT8030.10897074888552644No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAC737300.0144.113881
GCATACA737700.0144.03572
CATACAG249250.0143.71013
CATACAT258050.0142.832783
ATACAGG87600.0142.226524
ATACAGT90350.0142.206834
ATACATG99000.0141.944174
CATACAA221900.0141.634963
TACAGGT27800.0140.570885
TACAGTA28000.0140.081825
ATACAGA72100.0139.901814
TACAGTG29850.0139.854085
TACATGA25400.0138.240775
TACAATG28400.0138.108835
TACATGT40800.0138.016745
TACATAG26650.0137.979285
TACATGG31450.0137.78275
ATACAAG82500.0137.472444
ACATGTA21350.0137.44776
TACAGTT30000.0136.751435
Produced by FastQC (version 0.11.5)