FastQCFastQC Report
Sat 18 Mar 2023
SRR4895653_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895653_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences736895
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG201902.7398747447058267No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG36670.49762856309243514No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA32350.43900420005563884No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA28210.38282251881204243No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC14240.1932432707509211No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT296800.096.287831
AGCAGTG304450.093.660422
GGTATCA307350.092.9654168
GTATCAA308050.092.818469
CAGTGGT306350.092.771874
GTGGTAT307900.092.257766
AGTGGTA308350.092.1231165
TGGTATC308900.092.076457
GCAGTGG310700.091.4730153
CGCATAC1150.069.344751
CGGTCTG1350.064.44158145
TCGATCC350.003566759462.140095145
GGGCGCT1352.1420929E-532.22079145
TCGACAA900.003628407132.220787145
AAATATA19650.032.097813145
CGGTATC1255.151835E-429.0006797
TTGACCC2405.269976E-827.18629145
GCGGTAT1358.079579E-426.852486
AGCGGTA1358.079579E-426.852485
GGGTATC3108.56744E-1025.726417
Produced by FastQC (version 0.11.5)