FastQCFastQC Report
Sat 18 Mar 2023
SRR4895652_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895652_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences664261
Sequences flagged as poor quality0
Sequence length151
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTGGTCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT25770.38794991727649225No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTCA665750.0143.907842
AGTGGTC666400.0143.767491
TGGTCAG252000.0143.631853
TGGTCAT210450.0143.444663
GGTCAGT87600.0142.915634
TGGTCAA194950.0142.86413
GGTCAGA69400.0142.570194
GTCAGAT20600.0142.132345
GGTCATG91150.0142.096624
GTCAGTG32800.0141.814295
GTCATGA22150.0141.628165
GGTCATA59300.0141.314124
GGTCAAT60800.0141.26754
GGTCAGG94750.0141.264454
GTCAGTA25350.0141.10355
GGTCAAG78900.0140.850944
GGTCATT60850.0140.558854
GTCATTG30650.0140.468175
GTCATAG25600.0140.007255
GTCAGAG23200.0139.880975
Produced by FastQC (version 0.11.5)