FastQCFastQC Report
Sat 18 Mar 2023
SRR4895652_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895652_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences664261
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG188222.833524774147511No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA28370.42709115844524964No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT25030.3768097178669228No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23300.3507657381661726No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC19570.2946131114125321No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTGCCCCTCATTTACATAAAT11530.1735763502599129No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG10720.1613823482034923No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCTAAACCTAGCCCCAAACCC7570.11396122909518999No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACACGCGCTTCAACTTCGGT7090.10673515380249632No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC6890.10372428909720727No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT205500.082.128831
AGTGGTA213850.078.5152055
AGCAGTG216350.077.909522
GGTATCA216200.077.7623758
GTGGTAT215850.077.6197746
GTATCAA217200.077.4711159
CAGTGGT218400.076.912674
TGGTATC218650.076.69217
GCAGTGG221050.075.892233
CGGTCTG2250.057.99819145
CGCTGTT4700.041.64764145
CGGAGCC3450.039.926292145
CCGCAAG10150.033.57038145
CGTGCGA1204.057335E-430.207392145
AGCGCAT1504.3974465E-528.999094145
CGACCTA2550.025.0188345-49
GGTCTGA3501.2914825E-1024.856369145
TACGCAC2650.024.07472250-54
TGCGGCT4850.023.91678145
CATGGGG42100.023.66256525-29
Produced by FastQC (version 0.11.5)