FastQCFastQC Report
Sat 18 Mar 2023
SRR4895651_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895651_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences982661
Sequences flagged as poor quality0
Sequence length151
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACTGG983900.0144.025971
GACTGGA984050.0144.004012
ACTGGAT343000.0143.620573
ACTGGAG337800.0143.504793
ACTGGAA290450.0142.992743
CTGGATG122100.0142.791894
CTGGATA105250.0142.63354
CTGGAGT118700.0142.143834
CTGGAAT104050.0141.853044
CTGGAGA96100.0141.433044
CTGGAAG101000.0141.139374
CTGGATT116250.0141.045884
CTGGAGG124200.0139.913094
CTGGAAA89800.0136.982674
TGGATGA34100.0135.53975
TGGATAG37850.0132.969625
ACTGGAC18350.0132.81393
TGGAGTA39300.0131.916535
TGGATGG47350.0131.569955
TGGAATG38750.0129.695225
Produced by FastQC (version 0.11.5)