FastQCFastQC Report
Sat 18 Mar 2023
SRR4895651_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895651_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences982661
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG71180.7243596723590333No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA41210.4193714821286283No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT12350.1256791507956457No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC11500.117029168757079No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCGGGCGCGGTGGCTCAC10710.10898977368594051No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG10490.10675095480537032No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTTGTGGGGTTTTCTTCTA10230.10410507794651462No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGGGCGCGGTGGCTCACG10030.10206978805508715No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT153350.0103.487916
AGTGGTA154450.0102.9073945
GGTATCA155150.0102.894748
AAGCAGT156750.0102.245681
TGGTATC157050.0101.1421367
AGCAGTG158050.0101.119092
GTATCAA158600.0100.651379
CAGTGGT159250.099.992864
GCAGTGG161200.098.4683763
CGGTCTG3650.027.813215145
CGCCGAA1050.007701630727.62401145
AGCGCAT1855.8078786E-627.437363145
CGGAGCC4650.024.95072145
CGACCTA3250.024.53662745-49
CATGGGG57550.023.85833725-29
TACGCAC3350.023.37162650-54
CGGATCG2900.022.49831885-89
CAACGCA154950.021.8583210-14
AACGCAG156600.021.60949510-14
TACATGG153250.021.60882620-24
Produced by FastQC (version 0.11.5)