FastQCFastQC Report
Sat 18 Mar 2023
SRR4895650_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895650_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences551114
Sequences flagged as poor quality0
Sequence length151
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAACTTATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT11250.20413199446938382No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACTT551600.0144.054291
GAACTTA551750.0144.015142
AACTTAG188750.0143.459293
ACTTAGG69100.0142.411384
AACTTAT195050.0142.299973
ACTTAAG58250.0142.204774
ACTTATG72000.0142.082294
ACTTAGA56450.0141.630784
ACTTAGT63550.0141.575824
CTTATAG23050.0140.119055
AACTTAA166650.0140.075323
ACTTATA60350.0139.287254
CTTAGTA21200.0139.08415
CTTAGAG17200.0138.736135
CTTAGTG20250.0138.132785
CTTATGG26250.0137.593675
CTTAGGT25550.0136.56665
CTTAAGT19450.0136.030585
ACTTAAT58800.0135.970374
CTTAATG21850.0135.276545
Produced by FastQC (version 0.11.5)