FastQCFastQC Report
Sat 18 Mar 2023
SRR4895650_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895650_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences551114
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG127132.3067822628349126No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA29470.5347351001789103No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA17700.3211676712984972No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGGGCGCGGTGGCTCACG10570.19179334947034551No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT9530.17292248064828694No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACTGCGATAGGAATCATGTC8130.14751938800320805No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC8020.14552343072395185No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGGGCGCGGTGGCTCAC7550.13699524962167536No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT172300.090.219151
AGTGGTA174500.088.4905855
GTGGTAT176400.087.3239446
GGTATCA177600.087.305628
AGCAGTG177600.087.2727052
CAGTGGT177850.086.823774
GTATCAA179300.086.608199
TGGTATC178850.086.330487
GCAGTGG182500.084.571813
CGAACTT1258.913048E-1152.2025531
CGGTCTG2400.045.314713145
AGGGGTA1306.4636406E-427.8961035
GCTATCC2603.7107384E-927.885977145
GAGGTAT2156.0990715E-726.9854096
CGCGAAA1750.025.68463790-94
CGATAGG2200.025.04440735-39
CCGCAAG4950.024.900208145
CATGGGG45400.024.20823525-29
GGCTATC2401.5952319E-624.167847145
CTAAGCG2550.023.88135190-94
Produced by FastQC (version 0.11.5)