FastQCFastQC Report
Sat 18 Mar 2023
SRR4895649_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895649_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1134134
Sequences flagged as poor quality0
Sequence length151
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCTGAGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACT32600.2874439881001716No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCTGA1136300.0143.937992
TCCTGAT395850.0143.506793
TCCTGAG396650.0143.380973
TCCTGAA327200.0143.247543
CTGATAG43600.0142.894125
CCTGATG141400.0142.891334
CCTCCTG1145700.0142.757051
CCTGAAT117100.0142.493014
CCTGATA119200.0142.402424
CCTGAGT148950.0142.32924
CTGAGAT34750.0141.934655
CCTGAGA112050.0141.707414
CCTGAAG115000.0141.58374
CTGATGA37750.0141.5435
CTGAGTA41450.0140.911885
CCTGATT135050.0140.906784
CTGATGT49700.0140.590035
CCTGAGG136150.0140.562794
CTGAATG42550.0140.319465
CTGATGG50150.0139.472295
Produced by FastQC (version 0.11.5)