FastQCFastQC Report
Sat 18 Mar 2023
SRR4895649_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895649_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1134134
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG219711.937249037591678No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA41650.36724055534883887No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG28670.2527919981236785No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA27580.24318114085284454No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC15310.134992866804099No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC14300.12608739355314275No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACACGCGCTTCAACTTCGGT13900.12256047345375413No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT11380.1003408768276059No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT276600.085.163741
GGTATCA279600.084.2277458
GTGGTAT279600.083.812836
GTATCAA281650.083.603639
AGTGGTA280400.083.577395
TGGTATC282400.083.135877
AGCAGTG285050.082.59922
CAGTGGT285200.082.2506564
GCAGTGG290450.080.788913
CGGAGCC6900.038.876812145
CGGTCTG4400.032.954548145
TACGCAC2850.022.89372850-54
CATGGGG69250.022.27968225-29
CCGCAAG11200.022.008926145
GGTCTGA7350.021.70068145
ATGGGAG31650.019.6090825-29
CGACCTA3550.019.19650545-49
ATAGGCG4100.019.099245100-104
ATGGGGC25800.018.99696225-29
CGCGAAA3300.018.89377290-94
Produced by FastQC (version 0.11.5)