FastQCFastQC Report
Sat 18 Mar 2023
SRR4895648_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895648_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences878270
Sequences flagged as poor quality0
Sequence length151
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGAGA878800.0144.111852
CCGTGAG879550.0143.988971
GTGAGAG295100.0142.49183
GTGAGAT309400.0142.407733
GTGAGAA269350.0142.248023
TGAGATA100050.0140.598864
TGAGAGT104600.0140.410954
TGAGATG110000.0140.334874
TGAGAAT95200.0139.354984
TGAGAAG100550.0138.322164
TGAGAGG108900.0137.581254
TGAGAGA86100.0137.502824
TGAGATT103500.0135.354924
TGAGAAA83450.0131.501254
GTGAGAC16900.0131.40313
GAGATGA37050.0125.520065
GAGATTG39350.0124.9629445
GAGATTA34350.0124.051595
GAGATAG37600.0123.492225
GAGAGTG40700.0123.475265
Produced by FastQC (version 0.11.5)