FastQCFastQC Report
Sat 18 Mar 2023
SRR4895648_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895648_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences878270
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG94441.0752957518758468No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA45120.513737233424801No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT12690.1444885969007253No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA12390.14107279082742208No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC11320.12888974916597404No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG11000.12524622268778393No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT185350.098.058316
AGTGGTA185600.098.004355
AAGCAGT186700.097.9317251
GGTATCA186400.097.861558
GTATCAA188200.096.958599
AGCAGTG190050.095.982082
TGGTATC189650.095.916927
CAGTGGT191750.094.904264
GCAGTGG195850.092.7694243
GCCCCAA10200.027.00731145
CGGTCTG3057.1122486E-1026.145128145
TACGCAC3550.024.5047850-54
CATGGGG50700.023.33884625-29
CGACCTA3500.023.19785745-49
TAGTATC2653.7783393E-621.8872667
GGTCTGA5350.021.680243145
CGATAGG555.2178802E-621.09064535-39
ACATGGG174750.020.72612220-24
CGTGAGA2455.1122417E-520.7147332
CGGAGCC4901.8189894E-1120.712376145
Produced by FastQC (version 0.11.5)