FastQCFastQC Report
Sat 18 Mar 2023
SRR4895647_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895647_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1027005
Sequences flagged as poor quality0
Sequence length151
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAGTA1027550.0144.140582
CCGAAGT1027650.0144.126561
GAAGTAG342650.0143.14453
GAAGTAT361500.0142.562163
GAAGTAA319200.0142.094253
AAGTATG134200.0139.811724
AAGTAGT127450.0138.333634
AAGTAGG120200.0137.438814
AAGTAGA105750.0136.989844
AAGTAAG114800.0136.617454
AAGTATA116450.0134.805534
AAGTAAT117650.0133.491854
AGTATGG49450.0131.968735
AGTAGAG35650.0131.67715
AGTAATG40900.0131.523795
AGTAGTG43400.0130.925815
AGTAGGT45350.0130.702325
AGTAAGG42850.0130.586915
AGTATAG42050.0130.327595
AAGTATT128800.0130.221474
Produced by FastQC (version 0.11.5)