FastQCFastQC Report
Sat 18 Mar 2023
SRR4895647_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895647_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1027005
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG137091.3348523132798769No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA45680.4447884869109693No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA18810.18315392816977522No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT14460.14079775658346355No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG14060.13690293620771077No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC13640.13281337481317032No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCTTTTACCTCGTTGCACTGC10720.10438118607017492No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT204750.096.593221
AGTGGTA204650.095.542245
GGTATCA205450.095.346648
GTATCAA206750.095.097789
GTGGTAT205500.095.076496
AGCAGTG209400.094.136642
CAGTGGT208100.093.993124
TGGTATC208300.093.7984547
GCAGTGG217350.090.126373
CGAAGTA2450.047.345652
CCGAAGT4200.034.5228731
CGGTCTG3750.030.932493145
CATGGGG61700.023.75913425-29
GGTCTGA7150.021.293127145
CGACCTA2850.020.85908145-49
CCGCGAA3350.020.77656790-94
CGCGAAA3300.020.6519690-94
TACATGG200000.020.4307420-24
TACGCAC3200.020.39086550-54
ACATGGG194700.020.37620220-24
Produced by FastQC (version 0.11.5)