FastQCFastQC Report
Sat 18 Mar 2023
SRR4895646_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895646_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences575120
Sequences flagged as poor quality0
Sequence length151
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAGTTCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT68941.198706356934205No Hit
CCAGTTCATGTACTCTGCGTTGATACCACTGCTTCCCACGTACTCTGCGT7510.1305814438725831No Hit
CCAGTTCATGTACTCTGCGTTGATACCACTGCTCCCCATGTACTCTGCGT7020.1220614828209765No Hit
CCAGTTCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTT6020.10467380720545277No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTTCA576500.0143.848242
CCAGTTC576900.0143.748491
AGTTCAT209650.0143.373033
AGTTCAG197100.0143.28393
AGTTCAA159600.0142.707633
GTTCATG88450.0142.568124
GTTCAAT58650.0141.616854
GTTCAAG57200.0141.173254
GTTCAGT74750.0141.111884
GTTCAGA50900.0140.940484
GTTCATA54550.0140.629794
GTTCAGG72550.0140.52144
GTTCATT67350.0139.059944
TTCAGAT18200.0137.463855
TCAGTAG7150.0137.139726
TTCATGA19450.0136.784595
TTCAGTA23000.0136.6755
TCATGTA24900.0136.380426
TCATGAG7200.0135.185996
GTTCAAA46300.0134.855094
Produced by FastQC (version 0.11.5)