FastQCFastQC Report
Sat 18 Mar 2023
SRR4895646_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895646_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences575120
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG260774.5341841702601196No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA36540.6353456669912366No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA34420.5984837946863263No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT20890.36322854360829043No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC11490.1997843928223675No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACTGCGATAGGAATCATGTC10460.18187508693837806No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA6880.11962720823480318No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT239050.075.897051
AGCAGTG251000.072.28992
AGTGGTA253350.071.2348565
CAGTGGT255300.070.731464
GTATCAA257450.070.285339
GGTATCA257900.070.140688
GTGGTAT257350.070.0933766
TGGTATC258650.069.797147
GCAGTGG260450.069.3606953
GCTATCC2850.038.159016145
CTACTAA18450.029.865376145
CACGCAG4300.028.663631145
CGCAAGG4050.028.642817145
CCGCAAG6250.027.840816145
GGTCTGA2954.820322E-1027.03469145
CGATAGG2950.026.54453535-39
CGACCTA1650.026.36303545-49
ATAGCTA2259.093892E-725.778536145
TACGCAC1850.025.07963850-54
TCGTCGA1751.2545682E-424.857874145
Produced by FastQC (version 0.11.5)