FastQCFastQC Report
Sat 18 Mar 2023
SRR4895645_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895645_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1020617
Sequences flagged as poor quality0
Sequence length151
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATACCAATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT146771.438051688341464No Hit
CATACCAATGTACTCTGCGTTGATACCACTGCTTCCCACGTACTCTGCGT14290.14001334486883912No Hit
CATACCAAATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG11550.11316683927467405No Hit
CATACCAAGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACT10960.10738602237666038No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATACCA1023800.0143.764451
ATACCAA1025650.0143.505132
TACCAAT381800.0143.119813
TACCAAG349650.0143.039723
ACCAATG155250.0142.310934
ACCAAGT126200.0141.815434
TACCAAA289550.0139.931853
CCAATGT78750.0139.911655
ACCAATA110400.0139.70854
CCAATAG39150.0139.242285
ACCAATT120000.0138.146164
CAATGTA52800.0137.933126
ACCAAGG127550.0137.261674
ACCAAGA103450.0137.175234
CCAAGAT35950.0135.590385
CCAAGTG41700.0135.39635
CAAGATG12900.0135.271646
CCAATGA34650.0134.850595
CCAATTG45000.0134.761115
CCAATGG41050.0134.554035
Produced by FastQC (version 0.11.5)