FastQCFastQC Report
Sat 18 Mar 2023
SRR4895645_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895645_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1020617
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG642886.298934859991554No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA83350.8166628617787084No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA45190.44277138240887626No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG18600.18224270220856598No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA16690.16352853225058958No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC14670.14373658287094965No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT11390.11159916011589067No Hit
AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA11100.10875774164059583No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT521500.061.0272181
AGCAGTG536600.059.2017942
AGTGGTA538550.058.9980055
CAGTGGT540300.058.782964
GGTATCA545800.058.5378388
GTGGTAT542700.058.5248836
GTATCAA548350.058.3553059
TGGTATC546550.058.1922157
GCAGTGG546050.058.124123
CGGTCTG3200.031.717958145
CCGCAAG9750.026.02499145
AGCGGTA1801.5190577E-424.1696155
AGCGCAT1851.8310128E-423.512924145
CGACCTA2950.021.6265845-49
TACGCAC3100.021.51580250-54
CGATAGA3350.020.775396105-109
CATGGGG56200.020.5380625-29
TGCGGCT5700.020.35037145
CGATAGG1002.5465852E-1120.30068635-39
CGGTATC2155.057085E-420.2320547
Produced by FastQC (version 0.11.5)