FastQCFastQC Report
Sat 18 Mar 2023
SRR4895644_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895644_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences246003
Sequences flagged as poor quality0
Sequence length151
%GC34

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTGA1650.0144.207526
CGAGTAC306.730261E-10144.207526
ATGCGAT100.007204719144.207528
TCGAGAT8200.0144.207525
CGATACG202.1862052E-6144.207526
ACTTCGA246100.0144.148912
CACTTCG246150.0144.119641
CTTCGAG81750.0143.942933
TTCGAGA23300.0143.898064
CTTCGAT88550.0143.88183
CTTCGAA72200.0143.308723
TTCGAGG28800.0142.955724
TCGATGA8950.0142.596275
TTCGATG31100.0142.584614
TTCGAAG25600.0142.517584
TCGAGGA7900.0142.382115
TCGAAAT7650.0142.322455
TTCGAAT25250.0142.208594
TTCGAGT29700.0142.022574
CGATAGT3200.0141.954276