FastQCFastQC Report
Sat 18 Mar 2023
SRR4895644_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895644_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences246003
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG45371.8442864517912383No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18310.7442998662617936No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA6570.26706991378153927No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC5220.21219253423738735No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCCAGGCGCTGTGGCTCA4570.18577009223464752No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG4470.18170510115730296No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCAGGCACGGTGGCTCAC3390.13780319752198145No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT2860.11625874481205514No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT67000.098.900071
AGTGGTA68150.096.912035
GGTATCA68600.096.699048
GTGGTAT68950.095.7876056
GTATCAA69350.095.548749
AGCAGTG69500.095.1338962
GCAGTGG69700.094.756893
TGGTATC69700.094.756897
CAGTGGT69950.094.521884
CGGCTAT350.003560854162.153664145
CAAGTGC900.003619872332.227825145
ACGTAAG200.006075866528.99914780-84
GTGATAT1306.4700696E-427.8837955
TAATCCG605.875336E-1026.5825555-59
CGGCGGT556.7702786E-926.36286235-39
CCGCGTC1409.963004E-425.89736145
CCACTAC1409.974834E-425.8920962
ATGGGGG7600.024.99268725-29
CATGGGG15250.024.81566425-29
TCTCGCA653.9930455E-822.30703570-74
Produced by FastQC (version 0.11.5)