FastQCFastQC Report
Sat 18 Mar 2023
SRR4895643_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895643_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences791255
Sequences flagged as poor quality0
Sequence length151
%GC34

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATGGA793300.0143.811861
AATGGAA803100.0142.056962
ATGGAAT308250.0141.65863
ATGGAAG250450.0140.98933
TGGAATG106450.0139.105154
TGGAATA91550.0138.750964
ATGGAAA242950.0138.457443
TGGAAGT98550.0138.112764
TGGAAGG94100.0134.531314
TGGAAAT95100.0134.253754
TGGAATT117650.0134.073784
TGGAAAG86350.0133.66494
TGGAAGA66300.0131.897254
GGAATGA31550.0130.931375
GGAATGT39800.0130.780365
GGAATTG41450.0129.400775
GGAATAG32450.0129.299475
GGAAGTA33150.0127.873995
GGAATAT35900.0125.307945
GGAAGTG32650.0125.195385
Produced by FastQC (version 0.11.5)