FastQCFastQC Report
Sat 18 Mar 2023
SRR4895643_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895643_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences791255
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG146571.8523737606713386No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA35670.45080283852866654No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA19540.24694946635408307No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG10940.13826136959640065No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC10410.13156314967993885No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT210400.090.453461
GTGGTAT209500.090.2940446
GGTATCA211050.090.146228
AGTGGTA210550.089.809325
GTATCAA212800.089.3935859
TGGTATC213300.088.787417
AGCAGTG215550.088.067992
CAGTGGT216050.087.662814
GCAGTGG219050.086.396023
CGGTCTG2250.045.108635145
CGGAGCC3450.029.418674145
CGATAGG351.2565727E-424.8573535-39
ACTATCG851.8189894E-1223.881947140-144
CATGGGG43000.023.47055625-29
CGACCTA2150.022.92955245-49
GCCCCAA7350.022.68583145
TACGCAC2350.021.59592250-54
GAGGTAT3055.333586E-721.3949726
CGCTGTT4109.385985E-1021.218348145
TGCGGCT5500.021.08975145
Produced by FastQC (version 0.11.5)