FastQCFastQC Report
Sat 18 Mar 2023
SRR4895642_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895642_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences557721
Sequences flagged as poor quality0
Sequence length151
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGCCTAATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT28820.5167458281111882No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCTA558150.0144.099241
TGCCTAA558400.0144.034742
GCCTAAG214550.0143.946663
GCCTAAT184550.0143.551283
GCCTAAA153600.0143.182743
CCTAATG82300.0142.112764
CCTAAAG67500.0142.0794
CCTAAGT72950.0142.040924
CCTAAGG83300.0141.705174
CTAAGTG28700.0140.949335
CTAATAG20600.0140.715155
CTAAGAG22600.0140.705865
CTAATGG31150.0140.280265
CCTAAGA60050.0140.012744
CTAATGT30150.0139.193085
CTAAATG21200.0139.113565
CTAAGGG31350.0138.46535
CTAAGGT27350.0138.415275
CTAATGA18550.0138.38475
CCTAAAT48900.0138.022234
Produced by FastQC (version 0.11.5)