FastQCFastQC Report
Sat 18 Mar 2023
SRR4895642_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895642_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences557721
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG134432.410344957424949No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG25260.45291462935768956No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA24640.44179795991185555No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC19510.3498164853035837No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA17130.30714281872118854No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT13680.245283932288725No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATTCTATAGAAGAAATGCAA10680.19149359626049584No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGAAGAAATGCAAACTATCA6300.11295970565928125No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT203650.0102.2956541
AGCAGTG210250.098.765222
GGTATCA212150.098.213648
GTATCAA213750.097.817659
TGGTATC212250.097.484237
GTGGTAT212250.097.450076
AGTGGTA212700.097.320795
CAGTGGT213050.097.194954
GCAGTGG216900.095.302583
TCGGATA553.216705E-452.730793145
AGGTATC2450.038.468517
GAGGTAT2550.036.9599386
AGAGGTA2900.032.502175
GCCTAAT2003.2480784E-729.0019363
AAACCGC5850.023.79432985-89
TAGTATC2151.8641482E-523.6041137
GTGCAAA1851.8284257E-423.515083145
ATGGGAG36200.023.51189225-29
GAAACCG5850.023.29861385-89
AAATATA12450.023.29473145
Produced by FastQC (version 0.11.5)