FastQCFastQC Report
Sat 18 Mar 2023
SRR4895641_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895641_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences654425
Sequences flagged as poor quality0
Sequence length151
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGACTATGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC17250.2635901745807388No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAGACT655400.0143.973531
AAGACTA655750.0143.896682
AGACTAG223300.0143.870673
AGACTAT233900.0143.762013
GACTAGT77300.0143.633914
GACTATG90650.0143.239144
GACTAGG78950.0143.00644
AGACTAA186450.0142.994833
GACTAGA66100.0142.884664
GACTATA66650.0142.679124
GACTAAG66150.0142.66774
GACTAAT65800.0142.330844
GACTATT76950.0141.94494
ACTAGTG26550.0140.934945
ACTAGGT28600.0140.916415
ACTAATG23800.0140.255485
ACTAGTA22750.0139.756825
ACTATAG24300.0139.74315
ACTATGA23250.0138.921955
ACTAGAG21350.0138.79055
Produced by FastQC (version 0.11.5)