FastQCFastQC Report
Sat 18 Mar 2023
SRR4895641_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895641_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences654425
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG309204.724758375673301No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA34710.5303892730259387No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA25040.3826259693624174No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG13200.20170378576613057No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC11980.18306146617259428No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT11510.17587958895213357No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC9470.14470718569736793No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA8320.1271345073919853No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCTAAACCTAGCCCCAAACCC6770.10344959315429576No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT270300.069.058661
GGTATCA277500.067.240748
AGTGGTA276650.067.085475
GTATCAA278350.067.0409559
GTGGTAT278900.066.539186
AGCAGTG280300.066.537642
CAGTGGT281550.065.984584
TGGTATC281900.065.856797
GCAGTGG286900.064.728843
TTCGATA850.002741763634.11646145
CGGTCTG2206.220944E-1032.9534145
TGCGGCT3350.032.46156145
CGCTGTT3600.028.193462145
CGCAAGG4900.028.111265145
AGGGGTA1658.3943465E-526.3727955
AGCCGAC1450.001226238624.999132145
AAATATA12400.023.386282145
CATGGGG42950.021.63952625-29
TACGCAC1750.021.54210950-54
CGACCTA1850.021.16142745-49
Produced by FastQC (version 0.11.5)