FastQCFastQC Report
Sat 18 Mar 2023
SRR4895640_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895640_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences584284
Sequences flagged as poor quality0
Sequence length151
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCACATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT396286.782318187730624No Hit
CAACCACATGTACTCTGCGTTGATACCACTGCTTCCCACGTACTCTGCGT72931.248194371230429No Hit
CAACCACATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTT38960.6667990223932196No Hit
CAACCACATGTACTCTGCGTGGATACCACTGCTTCCCATGTACTCTGCGT15910.27229908743008535No Hit
CAACCACATGTACTCTGCGTTGATACCACTGCTCCCCATGTACTCTGCGT12920.22112534315504104No Hit
CAACCACATGTACTCTGCGTTGAGACCACTGCTTCCCATGTACTCTGCGT12680.21701775164132514No Hit
CAACCACATGTACTCTGCGTTGATACCACTGCTTCGCACGGACGCGCCGC9570.1637902116094228No Hit
CAACCACAATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG7750.13264097596374366No Hit
CAACCACATGTACTCTGCGTTGATACCACTGCTTCCCACGTACTCTGCGC7560.12938913268205188No Hit
CAACCACATGTACTCCGCGTTGATACCACTGCTTCCCATGTACTCTGCGT7250.12408349364350213No Hit
CAACCACATGTACTCTGCGTTGATACCACTGCTTAGATCGGAAGAGCGTC6950.11894900425135721No Hit
CAACCACATGTACCCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT6870.11757980708011857No Hit
CAACCACATGTACTCTGCGTTGATACCACTGCGTCCCATGTACTCTGCGT6710.11484141273764127No Hit
CAACCACATGTACTCTGCGTTGATACGACTGCTTCCCATGTACTCTGCGT6540.11193186874875916No Hit
CAACCACATGTACTCTGCGTTGATACCACTGCCTCCCATGTACTCTGCGT6470.11073382122392536No Hit
CAACCACATGTACTCTGCGTTGATACCACTGCTTCGCACGGACGCGCGGC6370.10902232475987704No Hit
CAACCACATGTACTCTGCGTTGATACCACTGCTTAGAACGGAAGAGCGGC6020.10303208713570797No Hit
CAACCACATGTACTCTGCGTTGATACCACTGCTTCCCAGGAACCCGCCGT6010.10286093748930315No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACATGT127100.0143.038775
CCACATG167000.0142.978014
ACCACAT283650.0142.908253
ACATGTA112500.0142.691856
CAACCAC591300.0142.510671
CCACAGT57750.0142.23234
AACCACA595550.0141.493652
CACATAG18300.0141.471795
CCACATT61150.0139.513054
CACAGTG18300.0139.107365
CCACATA56350.0138.215364
CACATTG19950.0137.723665
CCACAAT53600.0137.503134
CACAGTA19450.0137.185645
CACAATG19450.0137.185645
CACAGAT15800.0136.471075
CACAGTT19500.0136.464055
CCACAGA48350.0136.325234
CACATGA17150.0135.399875
CACATTA18950.0135.096975
Produced by FastQC (version 0.11.5)