FastQCFastQC Report
Sat 18 Mar 2023
SRR4895640_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895640_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences584284
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG8084413.836422013952118No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA119602.046949771001773No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG61321.049489631754421No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC25200.431297108940173No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20090.34383963962730457No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA19620.3357956062462775No Hit
AAGCAGTGGTATCAACGCAGAGTACATGTGGTTGAGATCGGAAGAGCACA14050.2404652531987869No Hit
AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA13140.22489063537594733No Hit
AGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG10950.18740886281328945No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA8450.14462145121208178No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT8030.13743316606307893No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC7640.13075832985329053No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG7490.12819108515721805No Hit
AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAG7350.12579499010755044No Hit
CAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT7210.12339889505788282No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT7150.12237199717945384No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG6960.11912015389776204No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC6600.11295876662718815No Hit
CAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA6390.10936462405268671No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGAAGAAATGCAAACTATCA6330.10833772617425771No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC6200.10611278077099492No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA6100.10440128430694662No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA6040.10337438642851764No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG5970.10217633890368383No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACTCG301.5991624E-596.663475145
AAATATA16050.059.1725145
AAGCAGT528150.055.5752261
AGCAGTG550100.053.339922
CAGTGGT567300.051.6588024
GCAGTGG572500.051.126273
AGTGGTA579050.050.610555
GTGGTAT584100.050.1481556
TGGTATC587550.049.9030577
GGTATCA591100.049.848678
GTATCAA593800.049.6543859
CATGCGG7900.044.04918145
CGTGCGG2900.042.498592145
CGTGCGC4800.039.269535145
CGGGCTC2500.037.69875145
CATGCGT2050.035.36468145
CAGGGGG4100.035.36468145
CGTGGGG5700.033.06908145
CCGGCGT5500.031.63532145
CCGGCTT6200.031.571539145
Produced by FastQC (version 0.11.5)