FastQCFastQC Report
Sat 18 Mar 2023
SRR4895639_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895639_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences800027
Sequences flagged as poor quality0
Sequence length151
%GC33

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTGAGG803500.0143.581531
TTGAGGA804450.0143.411962
TGAGGAG274600.0142.240333
TGAGGAT300250.0142.168413
TGAGGAA227050.0141.319783
GAGGATG103800.0134.970834
GAGGAGT104650.0134.908054
GAGGATA96150.0133.860784
GAGGAAT90000.0132.672914
GAGGATT117500.0132.36614
AGGAGTA31900.0129.969545
TGAGGAC11950.0128.52163
AGGATGT40050.0128.366425
AGGATTG40000.0128.346635
AGGATGA29650.0127.429815
AGGATTA32250.0127.217545
GAGGAGA85300.0126.965394
GAGGAAG80850.0126.016264
AGGATAT34350.0125.737425
AGGATAG33150.0125.721265
Produced by FastQC (version 0.11.5)