FastQCFastQC Report
Sat 18 Mar 2023
SRR4895639_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895639_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences800027
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG90141.1267119734708955No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA36450.4556096231752178No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA11960.1494949545452841No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACTGCGATAGGAATCATGTC9700.12124590795060666No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT154200.0100.0188751
GGTATCA155800.099.072418
AGTGGTA155400.098.814235
GTATCAA156450.098.60839
GTGGTAT155800.098.5146
TGGTATC157200.097.9594967
AGCAGTG159100.096.795692
CAGTGGT159100.096.567834
GCAGTGG164950.093.011163
CGGTCTG2150.040.468254145
GCTATCC3900.026.02766145
CGATAGG3050.025.19710435-39
TCGTAAG3300.025.045832100-104
GGTCGCG3950.024.226750-54
CGGAGCC3301.7698767E-924.168541145
CGTAAGG3250.024.09267100-104
CATGGGG53950.024.0012925-29
GTCGCGG4100.022.98671350-54
TGCGATA3350.022.94035730-34
TACGCAC2700.022.55448750-54
Produced by FastQC (version 0.11.5)