FastQCFastQC Report
Sat 18 Mar 2023
SRR4895638_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895638_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences742146
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG250083.369687366097776No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA38910.5242903687414605No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA32100.43252944838347174No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC11980.16142376297925207No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT248350.070.895981
GTATCAA253700.069.4108059
AGTGGTA255350.068.696895
AGCAGTG256750.068.468142
GTGGTAT257250.068.1475456
TGGTATC258700.067.79367
GGTATCA259400.067.7783668
CAGTGGT259600.067.576794
GCAGTGG263600.066.4963153
CGGTCTG1950.044.61354145
CCGCAAG6900.027.317713145
CGGAGCC2901.1901648E-824.998966145
CATGGGG46400.022.43838725-29
CGACCTA1750.020.71426645-49
ATAGGCG2550.020.47112100-104
ATGGGGC16800.020.45616525-29
CGATAGA2800.020.195866105-109
ATGGGGT8150.019.57132525-29
TACGCAC1800.019.33357850-54
CGTTCGA456.873771E-419.332796105-109
Produced by FastQC (version 0.11.5)