FastQCFastQC Report
Sat 18 Mar 2023
SRR4895637_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895637_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences428402
Sequences flagged as poor quality0
Sequence length151
%GC34

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACT428700.0144.09751
GTCACTA428800.0144.06392
TCACTAA193800.0143.565963
TCACTAG123600.0143.381743
TCACTAT106950.0142.917543
CACTAAG56250.0142.916664
CACTAGA74550.0142.844454
CACTATG33750.0142.275764
CACTATA62300.0141.76814
CACTAGG34650.0141.701484
ACTAGGA19150.0141.562935
CACTAAA115500.0141.326924
ACTAAGA35450.0141.147665
ACTAGAG20650.0140.706925
ACTATAG22250.0140.633975
ACTAAAG37100.0140.311665
CACTAGT14350.0140.178944
ACTAGAA45550.0140.0835
ACTATGA17100.0139.982095
CTATAGG7850.0139.606116
Produced by FastQC (version 0.11.5)