FastQCFastQC Report
Sat 18 Mar 2023
SRR4895637_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895637_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences428402
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG122682.863665435735594No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21200.49486230223014827No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA15920.37161357790113025No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT4610.10760920817363132No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG4580.1069089313308528No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT127650.079.142011
AGTGGTA129200.077.622185
CAGTGGT129550.077.421494
AGCAGTG129950.077.4064252
GGTATCA130600.077.280788
GTGGTAT129700.077.2586
GTATCAA131600.076.9049459
TGGTATC132100.075.964157
GCAGTGG132650.075.448153
CGGTCTG1550.051.45004145
CGAGCCG1504.3938286E-528.999113145
CGATAGG459.1125185E-725.77879335-39
TACGCAC1350.023.62890650-54
CTCGTTG1050.023.47711835-39
CATGGGG28450.023.19072225-29
TAACCCG452.6520831E-522.55697115-119
CGACCTA1350.022.55486545-49
CCTCGTT1100.022.40997335-39
TCTCGCA1500.021.26601670-74
CGCGAAA1100.021.09026390-94
Produced by FastQC (version 0.11.5)