FastQCFastQC Report
Sat 18 Mar 2023
SRR4895636_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895636_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences419622
Sequences flagged as poor quality0
Sequence length151
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCAGGA420650.0143.834531
GCAGGAA420950.0143.732032
CAGGAAT154000.0143.398593
CAGGAAG131050.0142.4893
CAGGAAA132050.0139.771993
AGGAATG55100.0138.437164
AGGAAGT50050.0137.280044
AGGAATA49100.0135.237384
AGGAATT55950.0135.04544
GGAATTG19900.0134.412435
AGGAAAT52650.0133.51344
GGAAGTA15600.0130.791785
GGAATAG15000.0130.736335
GGAATGT19300.0130.372715
GGAATGA15700.0129.958715
GGAAGTG16700.0127.789115
AGGAAGG53300.0127.286124
GGAATGG21300.0125.91635
CAGGAAC10250.0125.9063953
AGGAAAG49250.0125.4565054
Produced by FastQC (version 0.11.5)