FastQCFastQC Report
Sat 18 Mar 2023
SRR4895636_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895636_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences419622
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG164833.9280590626802216No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA24560.5852886645600088No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA23710.5650323386285752No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCCGGGCACGGTGGCTCA7050.16800835037247808No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT6780.16157398801778744No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGGGCACGGTGGCTCAC5760.1372663969000672No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACTGCGATAGGAATCATGTC5370.1279723179432918No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCCAGGCATAGTGGCTCA5370.1279723179432918No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA4610.10986077946342183No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCAGGCATAGTGGCTCAC4610.10986077946342183No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT161650.083.228711
AGTGGTA163400.081.95765
GTATCAA167150.080.6635749
GGTATCA167550.080.446918
AGCAGTG167300.080.177082
CAGTGGT167500.080.0380554
GTGGTAT167800.079.938176
GCAGTGG169100.079.109243
TGGTATC169650.079.109197
CCGCGAC502.008061E-458.0048646
CGCGACT400.00604644154.3795627
AGGGGTA1153.1574408E-431.5243855
CCGCAAG2603.7034624E-927.886953145
CGGTCTG1150.002750637525.219507145
GGTCTGA2051.287106E-524.758171145
CATGGGG34400.023.56279225-29
ATGGGGG15700.022.35059525-29
ATGGGGT3900.022.30796825-29
GCTATCC2303.134388E-522.06707145
CGACCTA1250.022.0392245-49
Produced by FastQC (version 0.11.5)