FastQCFastQC Report
Sat 18 Mar 2023
SRR4895635_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895635_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences629728
Sequences flagged as poor quality0
Sequence length151
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT41820.6640962447278825No Hit
AGATCGCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTT9610.15260556938868844No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGC630500.0144.024411
GATCGCA630650.0143.990172
ATCGCAT235650.0143.717833
ATCGCAG209050.0143.517583
ATCGCAA173450.0143.459143
TCGCAAG53750.0143.402534
TCGCAGT80150.0143.307794
TCGCATA70350.0143.284974
TCGCAAT68550.0143.260744
TCGCAGA57850.0142.836364
TCGCATT77250.0142.807334
TCGCATG87050.0141.639664
CGCATTG28950.0141.467715
CGCAGTT25550.0141.385335
CGCAGTA22750.0141.354935
CGCATAG24300.0141.240175
CGCAGTG26450.0140.663565
CGCAAGT20300.0140.300315
CGCAATG25500.0140.248765
CGCATAT25400.0139.949315
Produced by FastQC (version 0.11.5)