FastQCFastQC Report
Sat 18 Mar 2023
SRR4895635_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895635_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences629728
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG205483.262996087199553No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA39030.6197914020021342No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT32760.5202246049087861No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21350.3390352660196148No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC16430.26090629605162863No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG14920.23692768941511255No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCTAAACCTAGCCCCAAACCC11840.18801768382539763No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACAACCTTAGCCAAACCAT11440.1816657350475126No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAGGTTTGGTCCTAGCCTT10750.17070862340566084No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGTTCGTTTGTTCAACGAT10640.16896183749174248No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCGCGGTGGCTCACGCTT9640.15308196554702982No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCGTAAAGACGTTAGGTCAAG9200.14609482189135628No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGCAATAGATATAGTACC8050.12783296915493675No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTCATTATTACCCTCACTGTC6960.11052390873519996No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATCTTAGTTCAACTTTAA6890.10941231769907007No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGCGGTGGCTCACGCTTG6360.10099598556837237No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCGCAATAGATATAGTACCG6350.10083718684892524No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT257800.086.923971
AGCAGTG266200.083.990432
AGTGGTA265150.083.967595
CAGTGGT266050.083.792544
GTGGTAT266550.083.472176
GGTATCA267650.083.460788
GTATCAA268700.083.134649
TGGTATC267950.083.096757
GCAGTGG269200.082.731263
CGGAGCC2600.055.767372145
CGCGATC450.00963876448.33172145
CGGTCTG1406.6061693E-736.24879145
AGAGAGT16650.034.39825145
CACGCAG7850.033.247295145
CGCAAGG7600.032.43313145
CGAGCCG1902.0751213E-730.525295145
AGGGGTA1855.813161E-627.4315175
AGTGATA3451.07320375E-1025.2165495
CGACCTA2050.024.75527245-49
CATGGGG45750.024.50058225-29
Produced by FastQC (version 0.11.5)