FastQCFastQC Report
Sat 18 Mar 2023
SRR4895634_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895634_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences455834
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG114142.5039817126410053No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA29850.6548436492231822No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG17280.3790853687965356No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA14870.32621524502340765No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC12690.2783908177099558No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATTCTATAGAAGAAATGCAA6240.1368919387320823No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGAAGAAATGCAAACTATCA4680.10266895404906172No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT174900.0100.1885151
GTATCAA180900.096.825439
GGTATCA180400.096.793548
GTGGTAT180450.096.605976
AGCAGTG181000.096.472672
AGTGGTA181300.096.273045
CAGTGGT181250.096.259594
TGGTATC181850.095.7824867
GCAGTGG184100.094.7300343
CGGTCTG450.00963640248.332565145
CGGGGGG1050.00770472227.618607145
AGAGGTA1100.00965658226.3690035
GAAACCG4250.025.24665885-89
CGCTCGT300.001494996724.16628365-69
CCGCGAA900.005163695724.1662839
CATGGGG25450.024.0428425-29
ATGGGAG30250.023.58309725-29
AAACCGC4500.022.87741385-89
AAATATA11250.022.555197145
ATGGGAT29750.021.49377625-29
Produced by FastQC (version 0.11.5)