FastQCFastQC Report
Sat 18 Mar 2023
SRR4895633_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895633_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences620549
Sequences flagged as poor quality0
Sequence length151
%GC34

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTATC621000.0144.088651
CGTATCA621450.0143.98432
GTATCAG209700.0143.792163
GTATCAA180450.0143.645373
GTATCAT224150.0142.950243
TATCAGG77500.0142.530124
TATCAGT77100.0142.521444
ATCAGGT26750.0142.317965
TATCATG79700.0141.943074
TATCAGA55450.0141.474094
ATCAGTA23900.0141.187935
TATCAAG63550.0140.687584
ATCAGTG25350.0140.507195
TCAGGTA8600.0140.012746
ATCAGAG19300.0139.721715
ATCAGTC4700.0139.602465
ATCATGA21550.0139.520615
ATCAGAT19700.0139.446735
TATCAAT69250.0139.207054
ATCAGGA21600.0139.197655
Produced by FastQC (version 0.11.5)