FastQCFastQC Report
Sat 18 Mar 2023
SRR4895632_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895632_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences229453
Sequences flagged as poor quality0
Sequence length151
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG202.187291E-6144.188937
CGATCCA151.256545E-4144.188937
GTAACGC100.007207182144.188938
AACATCG229850.0143.9382
ACATCGT87650.0143.777663
CATCGGT26600.0143.646874
ACATCGG63450.0143.393573
AAACATC230850.0143.314481
CATCGGG22200.0143.214684
CATCGTT40000.0143.10754
ACATCGA64900.0142.633733
CATCGAG20150.0142.44
CATCGTA20000.0142.386574
ATCGATA6800.0142.06855
CATCGTG26800.0142.036854
CATCGGA13100.0141.987564
ATCGGTG9300.0141.86335
CATCGAT27200.0141.803454
ATCGATG7250.0141.205725
CATCGAA16450.0141.121084
Produced by FastQC (version 0.11.5)