FastQCFastQC Report
Sat 18 Mar 2023
SRR4895632_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895632_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences229453
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG132405.7702448867524065No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA18520.8071369735850045No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12560.5473887898611044No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG7850.3421179936631903No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC5880.2562616309222368No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA3420.1490501322710969No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT2850.12420844355924743No Hit
AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA2690.11723533795592125No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC2300.10023839304781372No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT106450.065.3264851
AGTGGTA107400.064.3970265
CAGTGGT107900.064.179794
GGTATCA108250.064.159258
AGCAGTG109000.063.7982062
GTATCAA109050.063.7550549
GTGGTAT109550.063.199366
TGGTATC110500.062.6560177
GCAGTGG111550.062.0797733
CCGCAAG1502.5855115E-838.665215145
GCCCCAA1702.9992843E-629.85182145
CCGCGAA403.2561184E-728.99891390-94
CGCGAAA459.112464E-725.77680890-94
CATGGGG15200.023.75239825-29
CATGTGC3702.564775E-1023.51263145
CTCGTTG502.2761342E-623.20418535-39
ATGGGGG4200.023.13008125-29
AAATATA5600.022.008102145
CGGCGGT806.89397E-1021.75392535-39
ATGGGGC5450.021.54964825-29
Produced by FastQC (version 0.11.5)