FastQCFastQC Report
Sat 18 Mar 2023
SRR4895631_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895631_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences562505
Sequences flagged as poor quality0
Sequence length151
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCTCG562950.0144.110321
CGCTCGA563000.0144.097522
GCTCGAG191350.0143.999483
GCTCGAT194600.0143.929143
GCTCGAA166900.0143.879943
CTCGATA60200.0143.626664
CTCGAGT65600.0143.346184
CTCGATG68050.0143.271874
CTCGAAT58400.0143.114294
CTCGAGA56600.0143.078924
CTCGATT65550.0143.015474
CTCGAAG56500.0142.821644
TCGAAGT20100.0142.790535
TCGAGTA22600.0142.630195
TCGAGAT21400.0142.203755
CTCGAGG67650.0142.200264
CGAGTAT7300.0141.262076
TCGATAA19300.0141.236475
CTCGAAA52850.0141.223744
TCGAATG20200.0141.012655
Produced by FastQC (version 0.11.5)