FastQCFastQC Report
Sat 18 Mar 2023
SRR4895631_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895631_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences562505
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG80921.4385649905334175No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19340.3438191660518573No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC12530.22275357552377315No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG11690.20782037493000063No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA9810.17439844979155741No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT7130.12675442884952134No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT111500.094.939661
GTGGTAT110600.094.9171146
GGTATCA111750.094.264728
TGGTATC112400.093.4615867
AGTGGTA112750.093.179755
GTATCAA113350.092.80629
CAGTGGT114050.092.1812064
AGCAGTG114850.091.981022
GCAGTGG118100.088.958653
ATTCGAC450.00963756648.3325657
CGCTCGA657.331199E-444.618642
GCCCCAA6750.034.369823145
CGGTCTG1501.1328611E-633.832794145
CGAATTA1358.0790475E-426.8514238
AGCGGTA1100.00966461926.3655595
GCGGTAT1100.00966880826.3632166
CGACCTA2600.025.09575545-49
CATGGGG30550.024.39698425-29
TACGCAC2650.024.07508950-54
CGGAGCC2151.864085E-523.604275145
Produced by FastQC (version 0.11.5)