FastQCFastQC Report
Sat 18 Mar 2023
SRR4895630_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895630_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences328819
Sequences flagged as poor quality0
Sequence length151
%GC34

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTCCAATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT34761.0571165291543372No Hit
ACCTCCAATGTACTCTGCGTTGATACCACTGCTTCCCACGTACTCTGCGT4240.12894632001192147No Hit
ACCTCCAATGTACTCTGCGTTGATACCACTGCTCCCCATGTACTCTGCGT3720.11313214868970468No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCTCCA330150.0143.61731
CTCCAAT121700.0143.081273
CTCCAAG111250.0143.040363
CCTCCAA331550.0143.010862
TCCAAGT39600.0141.839944
TCCAATG49850.0141.603424
CCAATGT21750.0140.891865
TCCAAGA30350.0140.168214
CCAAGTG13950.0140.071995
CAATGTA13300.0139.869926
CTCCAAA94300.0139.695713
TCCAATA35900.0139.386674
TCCAAGG42400.0138.255024
CCAATTG14800.0137.873545
CCAATGG15100.0137.521885
TCCAAAT34100.0137.22924
CCAATAG13000.0136.996615
TCCAATT37850.0136.396554
CCAAGTT13000.0135.887335
CAAGTAG4200.0135.623236
Produced by FastQC (version 0.11.5)