FastQCFastQC Report
Sat 18 Mar 2023
SRR4895629_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895629_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1150853
Sequences flagged as poor quality0
Sequence length151
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCAGA1153300.0143.899722
ACAGCAG1153950.0143.818661
AGCAGAT399850.0143.665083
GCAGATA122350.0143.086364
AGCAGAG385600.0143.065433
GCAGATG137750.0142.845144
GCAGATT137250.0142.630044
GCAGAAT121600.0142.48654
GCAGAGT130550.0142.383474
AGCAGAA356800.0142.185243
GCAGAAG125500.0141.275994
CAGATTG49450.0140.852435
CAGATAG42050.0140.60525
CAGAATG43350.0140.546865
CAGAGTA39850.0139.863595
GCAGAGA119600.0139.744844
CAGATGT45050.0139.724645
CAGATGA42300.0139.603745
GCAGAGG136800.0139.304324
CAGAGAT38750.0138.996055
Produced by FastQC (version 0.11.5)