FastQCFastQC Report
Sat 18 Mar 2023
SRR4895629_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895629_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1150853
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG156371.3587313062571849No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA55420.48155585465737155No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA20640.17934523349202722No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA217850.089.195458
AAGCAGT218900.088.962471
AGTGGTA218850.088.5266955
GTGGTAT219750.088.1273046
GTATCAA221700.087.606189
AGCAGTG222950.087.260242
TGGTATC223050.086.888487
CAGTGGT223400.086.8248144
GCAGTGG229450.084.345853
CGGTCTG3709.094947E-1225.471264145
CATGGGG55150.022.29483625-29
CGACCTA3050.021.39256745-49
TACGCAC3100.021.04880350-54
TCTCGCA3600.019.73478770-74
ACATGGG196250.019.52055720-24
TACATGG205950.019.4389920-24
GGTCTGA6350.019.408146145
AGAGTAC213050.019.16533315-19
GTACATG211950.019.10761620-24
CAGAGTA215000.019.00499715-19
Produced by FastQC (version 0.11.5)